piggyBac transposon remobilization and enhancer detection in Anopheles mosquitoes
نویسندگان
چکیده
منابع مشابه
Gal4-based Enhancer-Trapping in the Malaria Mosquito Anopheles stephensi
Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human malaria vector, Anopheles stephensi. The system consists of six transgenic lines of Anopheles stephensi, each with a single piggyBac-Gal4 element in a uni...
متن کاملAnopheles Mosquitoes and Imported Malaria in Libya
Anopheles multicolor Cambouliu, An. sergentii Theobald and, in a circumscribed coastal area, An. labranchiae Falleroni are considered to have been vectors of malaria in Libya where the incidence of imported malaria has increased. Libyan records of the Afrotropical vector An. gambiae Giles sensu Zato are considered erroneus. However, two factors increase the risk of invasion from the south byAn....
متن کاملGeneration of an inducible and optimized piggyBac transposon system†
Genomic studies in the mouse have been slowed by the lack of transposon-mediated mutagenesis. However, since the resurrection of Sleeping Beauty (SB), the possibility of performing forward genetics in mice has been reinforced. Recently, piggyBac (PB), a functional transposon from insects, was also described to work in mammals. As the activity of PB is higher than that of SB11 and SB12, two hype...
متن کاملMembers of the piggyBac transposon family in Aedes Aegypti
piggyBac is a Class-II transposable element with 13-bp inverted terminal repeats. It was originally isolated from the cabbage looper moth, Trichoplusia ni. In this article, we analyze the Aedes aegypti genome to discover possible members of the piggyBac family of transposons using a combination of techniques. Specifically, we construct a set of Hidden Markov Models based upon multiple sequence ...
متن کاملBreeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Xenopus is a powerful model for studying a diverse array of biological processes. However, despite multiple methods for transgenesis, relatively few transgenic reporter lines are available and commonly used. Previous work has demonstrated that transposon based strategies are effective for generating transgenic lines in both invertebrate and vertebrate systems. Here we show that the Tol2 transpo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2011
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1110628108